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Early determination and long-term persistence of adult-generated new neurons in the hippocampus of mice
Abstract
New neurons are continually generated in the adult hippocampus, but the important question, whether adult neurogenesis is transient or leads to the lasting presence of new neurons, has not yet been answered. Dividing cells were labeled with bromodeoxyuridine (BrdU) and were investigated by means of immunofluorescence and confocal microscopy at several time-points 1 day to 11 months thereafter. BrdU-labeled neurons remained stable in number and in their relative position in the granule cell layer over at least 11 months. This finding implies that the addition of new neurons is not transient and that their final number and localization are determined early. By contrast,expression of immature markers β-III-tubulin and doublecortin in BrdU-labeled cells, peaked early after division and was not detectable after 4 weeks. In transgenic mice expressing enhanced green fluorescent protein under the nestin promoter none of the BrdU/nestin-positive cells early after division expressed the mature marker NeuN, confirming that no dividing neurons were detected. These new data suggest that new neurons are recruited early from the pool of proliferating progenitor cells and lead to a lasting effect of adult neurogenesis.
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Self-citationNam, S. M., Kim, J. W., Yoo, D. Y., Choi, J. H., Kim, W., Jung, H. Y., Won, M.-H., Hwang, I. K., Seong, J. K., & Yoon, Y. S. (2013) · Neurochemical Research · doi:10.1007/s11064-013-1169-y · Cited by 26
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Metadata
- Authors
- Kempermann, G., Gast, D., Kronenberg, G., Yamaguchi, M., & Gage, F. H.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2003-01-15
- Volume
- 130
- Issue
- 2
- Pages
- 391-399
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2042154782
- Cited by
- 983
- References
- 46
- FWCI
- 8.05
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.