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Cited by 1,636

The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.

Specificity of microRNA target selection in translational repression

Open access
Doench, John G., Sharp, Phillip A.·2004·Genes & Development

Abstract

MicroRNAs (miRNAs) are a class of noncoding RNAs found in organisms as evolutionarily distant as plants and mammals, yet most of the mRNAs they regulate are unknown. Here we show that the ability of an miRNA to translationally repress a target mRNA is largely dictated by the free energy of binding of the first eight nucleotides in the 5′ region of the miRNA. However, G:U wobble base-pairing in this region interferes with activity beyond that predicted on the basis of thermodynamic stability. Furthermore, an mRNA can be simultaneously repressed by more than one miRNA species. The level of repression achieved is dependent on both the amount of mRNA and the amount of available miRNA complexes. Thus, predicted miRNA:mRNA interactions must be viewed in the context of other potential interactions and cellular conditions.

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Crossref · 10.1101/gad.1184404

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Metadata

Authors
Doench, J. G., & Sharp, P. A.
Venue
Genes & Development
Published
2004-03-01
Volume
18
Issue
5
Pages
504-511
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
OpenAlex
W2109136664
Cited by
1,636
References
9
Type
posted content
Language
en
Open access
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